@article {12964,
	title = {Characterization of Pseudoalteromonas citrea and P. nigrifaciens Isolated from Different Ecological Habitats Based on REP-PCR Genomic Fingerprints},
	journal = {Systematic and Applied Microbiology},
	volume = {25},
	year = {2002},
	month = {2002///},
	pages = {275 - 283},
	abstract = {SummaryDNA primers corresponding to conserved repetitive interspersed genomic motifs and PCR were used to show that REP, ERIC and BOX-like DNA sequences are present in marine, oxidative, Gram-negative Pseudoalteromonas strains. REP, ERIC and BOX-PCR were used for rapid molecular characterization of both the type species of the genus and environmental strains isolated from samples collected in different geographical areas. PCR-generated genomic fingerprint patterns were found to be both complex and strain specific. Analysis of the genotypic structure of phenotypically diverse P. citrea revealed a geographic clustering of Far Eastern brown-pigmented, agar-digesting strains of this species. Marine isolates of P. nigrifaciens with 67{\textendash}70\% DNA relatedness generated genomic patterns different from those of the type strain and formed a separate cluster. It is concluded that REP, ERIC and BOX-PCR are effective in generating strain specific patterns that can be used to elucidate geographic distribution, with these genomic patterns providing a valuable biogeographic criterion.
},
	keywords = {biogeography, BOX-PCR, ERIC, Pseudoalteromonas, REP},
	isbn = {0723-2020},
	doi = {10.1078/0723-2020-00103},
	url = {http://www.sciencedirect.com/science/article/pii/S0723202004701125},
	author = {Ivanova,Elena P. and Matte,Glavur R. and Matte,Maria H. and Coenye,Tom and Huq,Anwarul and Rita R Colwell}
}
